The purpose of this study was to identify the motivating components of Khamsa ʻAshar supplication on students’ intimate relationship with Allah. The statistical population of the study consisted of female high school students in the Tabadakan region of Mashhad in the academic year 2019-2020, and consisted of about 6150 students, and 360 students were selected as a sample through simple random sampling according to Morgan's table. This research method was a mixed one in terms of implementation (qualitative-quantitative). In order to analyze data in the qualitative section, the text of the Khamsa ʻAshar supplication was studied using NVivo10 quality software, and then the components affecting human’s intimate relation with Allah were identified through open and central coding. Also, based on the extracted components, a 32-item researcher-made questionnaire with a 5-point Likert scale was developed. The face and content validity of the questionnaire were confirmed by expert professors. The reliability of the questionnaire was calculated to be 0.935 Cronbach’s alpha. In the quantitative part, for data analysis, descriptive and inferential statistics including Cronbach’s alpha, extracted mean-variance, the mean root of extracted variance, Kolmogorov-Smirnov, exploratory factor analysis, confirmatory factor analysis, SP1, and single sample t-test through the NVivo10, SPSS24, and Smart-PLS software were used. The results showed that the four components of ‘one’s own right’, ‘Allah’s right’, ‘people’s right’, and ‘Infallibles’ right’ extracted from the Khamsa ʻAshar supplication have a positive effect on the intimate relationship of female students with Allah. Thus, inclusion of the supplication in the textbooks can be effective in the students’ intimate relation with Allah.